Tim Kåre Jensen - histology

Pathology

The research in veterinary pathology is focused on pathogenesis and in the diagnosing of animal diseases applying various microscopy techniques to examine tissue sections. The research primarily concentrates on intestinal disorders in livestock and on developing in situ detection methods for bacteria and other microorganisms.

Pathology

The pathologists are primarily involved with the diagnosis of diseases in livestock as well as in wild animals; how the diseases appear in the cells and in different organs, what causes of the diseases in question, and how they develop, i.e. the pathogenesis of the disease.

When the causative infectious agent has been identified from diseases that has naturally infected an animal, the pathologists often develop and test infection models in order to study the pathogenesis. From those studies, an even more in-depth knowledge of the diseases can be achieved. For example, it can be established which cells and organs are the ways of entrance for the pathogen, the incubation time, the symptoms, as well as strategies for prevention of the disease.

Toxoplasmose med hjernenekrose i mus (cystozoiter - brune) IHC

Greater knowledge and diagnoses

The aim is to get more knowledge about the disease, so that we for instance can develop effective methods to determine which infectious agent that causes the disease, and how the disease can be prevented.

In addition to a gross evaluation of organs  and carcasses, microscopy of tissue sections are used for diagnosing and characterization of lesions both at organ level and at a cellular level.

Furthermore, we use microscopy to detect and identify for example bacteria directly in the tissue. The in situ detection is done by immunohistochemical methods (IHC) as well as fluorescence in situ hybridization (FISH) using oligonucleotide probes targeting ribosomal RNA.

Intestinal disorders and skin diseases

In terms of research, the work concentrates on intestinal disorders in mink and especially in pigs - for example revealing the cause of neonatal diarrhea and infections with Lawsonia intracellularis, porcine circovirus type 2 (PCV2), and Brachyspira spp. amongst growers and finishers. This work is done in collaboration with the institute’s other research groups. Reproduction pathology and diagnosis of abortion causes is another research area of interest, including in situ diagnostic of chlamydia.

In cattle, the research  specifically deals with the investigation of the bacteria causing endometritis and digital dermatitis, which are both diseases involving biofilm formation. In digital dermatits multiple species of Treponema appears to be the main cause. The Treponema attack the skin between the heel bulbs especially on the hind feet  causing lameness, and thus the disease has important implications for both the economy and animal welfare.

The pathologists also conduct consultative and diagnostic histopathology and FISH analyses on formalin-fixed tissue from Denmark and abroad when there is a suspicion of an outbreak of an infectious disease in livestock.

Senior Researcher

Tim Kåre Jensen
Professor
DTU Vet
+45 35 88 61 47