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| Titel:
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Time course study of in situ expression of antigens following DNA-vaccination against VHS in rainbow trout (Oncorhynchus mykiss Walbaum) fry |
| Type:
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Journal articleJournal article |
| Person(er):
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Technical University of Denmark
Email:
Technical University of Denmark
Email:
Technical University of Denmark
Forfatter:
Brudeseth, B.
Technical University of Denmark
Forfatter:
Evensen, O.
Technical University of Denmark
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| Uddrag:
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The present study was performed as a time course study of fish vaccinated with 20 mu g plasmid DNA vaccine encoding either the VHSV G-protein or the VHSV N-protein. Samples of the injection site were collected sequentially over a 7-week period. The study revealed an intense positive staining by immunohistochemistry for the viral G-protein mainly in the membrane of intact myocytes, most prominent by days 10-27, and with concomitant infiltration of inflammatory cells by days 13-38 that subsequently lead to a marked reduction in the number of myocytes expressing the G-protein. By immunofluorescence, infiltrating cells positive for MHC II, IgM, and C3 were demonstrated. By contrast, in fish vaccinated with the VHSV-N construct, fewer, diffusely positive myocytes were found, most prominent by days 13-38, these having a positive reaction for the N-protein mainly in the cytoplasm and variably in the membrane. N-protein positive myocytes did not attract infiltrating cells to the same degree. Positive reaction for the N-protein almost ceased by day 48 post-vaccination. |
| Publiceret:
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in journal: Fish & Shellfish Immunology (ISSN: 1050-4648) (DOI: http://dx.doi.org/10.1016/j.fsi.2004.10.009), vol: 19, issue: 1, pages: 27-41, 2005 |
| DOI:
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