Titel: Use of automated real-time reverse transcription-polymerase chain reaction (RT-PCR) to monitor experimental swine vesicular disease virus infection in pigs
Type: Journal articleJournal article
Person(er):
Forfatter:  Reid, S.M.
Technical University of Denmark

Forfatter:  Paton, D.J.
Technical University of Denmark

Forfatter:  Wilsden, G.
Technical University of Denmark

Forfatter:  Hutchings, G.H.
Technical University of Denmark

Forfatter:  King, D. P.
Technical University of Denmark

Forfatter:  Ferris, N.P.
Technical University of Denmark

Forfatter:  Alexandersen, Søren (Cwisno: 39440)
Technical University of Denmark
Uddrag: Automated real-time RT-PCR was evaluated as a diagnostic tool for swine vesicular disease virus (SVDV) infection on a range of samples (vesicular epithelium, serum, nasal swabs, faeces) from four inoculated and three in-contact pigs over a period of 28 days. Traditional diagnostic procedures (virus isolation, and ELISAs for antigen and antibody) were used in parallel. Each inoculated pig developed a significant viraemia and clinical disease, and excreted virus, which was transmitted to the in-contact animals. The latter, however, developed only a short-lived, low-level viraemia and no clinical disease. The RT-PCR and virus isolation were generally comparable in detecting SVDV in the serum and nasal swabs from inoculated and in-contact pigs up to day 6 after infection; it was possible, however, to isolate virus for a longer period from the faeces of a few pigs. This suggested that further optimization of the template extraction method was required to counteract the effects of RT-PCR inhibitors in faeces. It was concluded that the automated real-time RT-PCR is a useful diagnostic method for SVD in clinically or subclinically affected pigs and contributed to the study of the pathogenesis of SVD in the pigs.
Publiceret: in journal: Journal of Comparative Pathology (ISSN: 0021-9975), vol: 131, issue: 4, pages: 308-317, 2004
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