| Titel:
|
RT-PCR-ELISA as a tool for diagnosis of low-pathogenicity avian influenza |
| Type:
|
Journal articleJournal article |
| Person(er):
|
Forfatter:
Dybkær, Karen
National Veterinary Institute
Technical University of Denmark
Email:
Technical University of Denmark
Technical University of Denmark
|
| Uddrag:
|
A one-tube reverse transcriptase/polymerase chain reaction coupled with an enzyme-linked immunosorbent assay (RT-PCR-ELISA) was developed for the rapid detection of avian influenza virus (AIV) in clinical specimens. A total of 419 swab pools were analyzed from chickens experimentally infected with low-pathogenicity AIV, from wild aquatic birds, and from domestic ducks. The AIV was detected in 32 swab pools by RT-PCR-ELISA compared to 23 by virus isolation (VI) in embryonated specific pathogen free (SPF) chicken eggs. Thus, 39% more specimens were positive by RT-PCR-ELISA than by VI. Two of the twenty-three VI-positive specimens were negative when tested by RT-PCR-ELISA. The diagnostic sensitivity and specificity of the RT-PCR-ELISA was 91% and 97%, respectively, using VI in SPF eggs as the gold reference standard. |
| Publiceret:
|
in journal: Avian Diseases (ISSN: 0005-2086) (DOI: http://dx.doi.org/10.1637/0005-2086-47.s3.1075), vol: 47, issue: s3, pages: 1075-1078, 2003 |
| DOI:
|
|
|