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Titel: Comparison of methods and animal models commonly used for investigation of fecal microbiota: Effects of time, host and gender
Type: Journal articleJournal article
Person(er):
Forfatter:  Bernbom, Nete (Cwisno: 39772)
Technical University of Denmark

Forfatter:  Nørrung, Birgit (Cwisno: 39066)
Technical University of Denmark

Forfatter:  Saadbye, Peter
Technical University of Denmark, National Food Institute

Forfatter:  Mølbak, Lars (Cwisno: 14603)
Technical University of Denmark

Forfatter:  Vogensen, Finn K.
Royal Veterinary and Agricultural University, Department of Food Science

Forfatter:  Licht, Tine Rask (Cwisno: 2791)
Technical University of Denmark
Email:

Uddrag: Denaturing gradient gel electrophoresis (DGGE), terminal restriction fragment length polymorphism (T-RFLP) and plating on selective agars were used to study variation in the fecal microbiota of rats over time as well as variation between individuals. Investigated rats were either conventional and specific pathogen free (SPF). or human flora associated (HFA). A higher variation (p <0.05) in fecal microbiota over time was observed for HFA than for SPF animals. Analysis of DGGE and T-RFLP profiles of fecal microbiota from SPF and HFA rats revealed that variation over time was less significant than variation between individuals, and that phylogenetic profiles clustered according to gender. These observations should be taken into account when designing future research addressing changes in fecal microbiota.
Publiceret: in journal: Journal of Microbiological Methods (ISSN: 0167-7012) (DOI: http://dx.doi.org/10.1016/j.mimet.2005.10.014), vol: 66, issue: 1, pages: 87-95, 2006
DOI:
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