| Titel:
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Blocking enzyme-linked immunosorbent assay for detection of antibodies against Actinobacillus pleuropneumoniae serotype 6 in pig serum |
| Type:
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Journal articleJournal article |
| Person(er):
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Technical University of Denmark
Email:
Technical University of Denmark
Email:
Technical University of Denmark
Technical University of Denmark
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| Uddrag:
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A blocking enzyme-linked immunosorbent assay (ELISA) detecting antibodies against Actinobacillus pleuropneumoniae (Ap) serotype 6 was developed. The blocking ELISA was based on the inhibition of a polyclonal antibody raised against Ap serotype 6. Purified lipopolysaccharide from Ap serotype 6 was used as antigen. The blocking ELISA was tested against sera from pigs experimentally infected with the 12 serotypes of Ap biotype 1. Cross-reaction with serotypes 3 and 8 but not with other serotypes was observed. The sensitivity and specificity of the test on a herd level were evaluated with sera from herds naturally infected with serotypes 2, 6, 8 or 12 and with sera from herds free of infection with any Ap serotype. The blocking ELISA showed a high herd sensitivity (1.00 (0.79-1.00)) and specificity (0.97 (0.93-0.99)). |
| Publiceret:
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in journal: Veterinary Microbiology (ISSN: 0378-1135) (DOI: http://dx.doi.org/10.1016/S0378-1135(00)00349-7), vol: 79, issue: 1, pages: 11-18, 2001 |
| DOI:
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