Titel: Classical swine fever virus detection: results of a real-time reverse transcription polymerase chain reaction ring trial conducted in the framework of the European network of excellence for epizootic disease diagnosis and control
Type: Journal articleJournal article
Person(er):
Forfatter:  Hoffmann, Bernd
Friedrich-Loeffler-Institut, Institute of Diagnostic Virology

Forfatter:  Blome, Sandra
Friedrich-Loeffler-Institut, Institute of Diagnostic Virology

Forfatter:  Bonilauri, Paolo
Lombardy and Emilia Romagna Experimental Zootechnic Institute

Forfatter:  Fernández-Piñero, Jovita
Animal Health Research Center, Madrid

Forfatter:  Greiser-Wilke, Irene
University of Veterinary Medicine, Hannover, Institute of Virology

Forfatter:  Haegeman, Andy
Veterinary and Agrochemical Research Center, Belgium

Forfatter:  Isaksson, Mats
National Veterinary Institute, Uppsala, Sweden

Forfatter:  Koenen, Frank
Veterinary and Agrochemical Research Center, Belgium

Forfatter:  LeBlanc, Neil
National Veterinary Institute, Uppsala, Sweden

Forfatter:  Leifer, Immanuel
Institute of Virology and Immunoprophylaxis, Mittelhäusern, Switzerland

Forfatter:  Le Potier, Marie-Frederique
French Agency for Food Environmental and Occupational Health Safety, Swine Virology and Immunology Section

Forfatter:  Loeffen, Willie
Central Veterinary Institute, Lelystad, The Netherlands

Forfatter:  Rasmussen, Thomas Bruun (Cwisno: 39467)
Technical University of Denmark
Email:

Forfatter:  Stadejek, Tomasz
National Veterinary Research Institute, Poland, Department of Swine Diseases

Forfatter:  Ståhl, Karl
National Veterinary Institute, Uppsala, Sweden

Forfatter:  Tignon, Marylène
Veterinary and Agrochemical Research Center, Belgium

Forfatter:  Uttenthal, Åse (Cwisno: 39056)
Technical University of Denmark
Email:

Forfatter:  van der Poel, Wim
Central Veterinary Institute, Lelystad, The Netherlands

Forfatter:  Beer, Martin
Friedrich-Loeffler-Institut, Institute of Diagnostic Virology
Uddrag: The current study reports on a real-time reverse transcription polymerase chain reaction (real-time RT-PCR) ring trial for the detection of Classical swine fever virus (CSFV) genomic RNA undertaken by 10 European laboratories. All laboratories were asked to use their routine in-house real-time RT-PCR protocols and a standardized protocol commonly used by the Friedrich-Loeffler-Institute (FLI) on a panel of well-characterized samples. In general, all participants produced results within the acceptable range. The FLI assay, several in-house assays, and the commercial kits had high analytical sensitivity and specificity values. Nevertheless, some in-house systems had unspecific reactions or suboptimal sensitivity with only a single CSFV genotype. Follow-up actions involved either improvement of suboptimal assays or replacement of specific laboratory assays with the FLI protocol, with or without modifications. In conclusion, the ring trial showed reliability of classical swine fever diagnosis on an international level and helped to optimize CSFV-specific RT-PCR diagnostics.
Publiceret: in journal: Journal of Veterinary Diagnostic Investigation (ISSN: 1040-6387) (DOI: http://dx.doi.org/10.1177/1040638711416849), vol: 23, issue: 5, pages: 999-1004, 2011
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