| Titel:
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Bovine NK cells can produce gamma interferon in response to the secreted mycobacterial proteins ESAT-6 and MPP14 but not in response to MPB70 |
| Type:
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Journal articleJournal article |
| Person(er):
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Forfatter:
Olsen, Ingrid
Technical University of Denmark
Forfatter:
Boysen, P.
Technical University of Denmark
Forfatter:
Kulberg, S.
Technical University of Denmark
Forfatter:
Hope, J.C.
Technical University of Denmark
Technical University of Denmark
Email:
Forfatter:
Storset, A.K.
Technical University of Denmark
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| Uddrag:
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Bovine NK cells have recently been characterized and the present study describes the interaction between NK cells, antigen-presenting cells, and secreted mycobacteriall proteins. Gamma interferon (IFN-gamma) production by NK cells was seen in approximately 30% of noninfected calves in response to the Mycobacterium tuberculosis complex-specific protein ESAT-6, MPP14 from Mycobacterium avium subsp. paratuberculosis, and purified protein derivative (PPD) from M. tuberculosis. In contrast, no response was induced by MPB70, which is another M. tuberculosis complex-specific secreted antigen. The production of IFN-gamma by NK cells in whole blood in response to ESAT-6 and MPP14 was demonstrated using intracellular staining together with surface labeling for the NK cell-specific receptor, NKp46, or CD3. Furthermore, the depletion of NK cells from peripheral blood mommuclear cells completely abolished the IFN-gamma production. The response was mediated through stimulation of adherent cells and was largely independent of contact between adherent cells and the NK cells. Neutralization of interleukin-12 only partly inhibited IFN-gamma production, showing that other cytokines were also involved. The demonstration of NK cell-mediated IFN-gamma production in young cattle provides an explanation for the nonspecific IFN-gamma response frequently encountered in young cattle when using the IFN-gamma test in diagnosis of mycobacteriall infections. |
| Publiceret:
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in journal: Infection and Immunity (ISSN: 0019-9567) (DOI: http://dx.doi.org/10.1128/IAI.73.9.5628-5635.2005), vol: 73, issue: 9, pages: 5628-5635, 2005 |
| DOI:
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