Titel: Generation of recombinant pestiviruses using a full-genome amplification strategy
Type: Journal articleJournal article
Person(er):
Forfatter:  Rasmussen, Thomas Bruun (Cwisno: 39467)
Technical University of Denmark
Email:

Forfatter:  Reimann, I.
Technical University of Denmark

Forfatter:  Uttenthal, Åse (Cwisno: 39056)
Technical University of Denmark
Email:

Forfatter:  Leifer, I.
Technical University of Denmark

Forfatter:  Depner, K.
Technical University of Denmark

Forfatter:  Schirrmeier, H.
Technical University of Denmark

Forfatter:  Beer, M.
Technical University of Denmark
Uddrag: Complete genome amplification of viral RNA provides a new tool for the generation of modified viruses. We have recently reported a full-genome amplification strategy for recovery of pestiviruses (Rasmussen et al., 2008). A full-length cDNA amplicon corresponding to the Border disease virus-Gifhorn genome was generated by long RTPCR and then RNA transcripts derived from this amplicon were used to rescue infectious virus. Here, we have now used this full-genome amplification strategy for efficient and robust amplification of three additional pestivirus strains: the vaccine strain C and the virulent Paderborn strain of Classical swine fever virus plus the CP7 strain of Bovine viral diarrhoea virus. The amplicons were cloned directly into a stable single-copy bacterial artificial chromosome generating full-length pestivirus DNAs from which infectious RNA transcripts could be also derived.
Publiceret: in journal: Veterinary Microbiology (ISSN: 0378-1135) (DOI: http://dx.doi.org/10.1016/j.vetmic.2009.09.037), vol: 142, issue: 1-2, pages: 13-17, 2010
DOI:
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