Title: Application of the Ceditest FMDV type O and FMDV-NS enzyme-linked immunosorbent assays for detection of antibodies against Foot-and-mouth disease virus in selected livestock and wildlife species in Uganda
Type: Journal articleJournal article
Participant(s):
Forfatter:  Ayebazibwe, Chrisostom
Ministry of Agriculture, Animal Industry and Fisheries

Forfatter:  Mwiine, Frank Norbert
Ministry of Agriculture, Animal Industry and Fisheries

Forfatter:  Balinda, Sheila Nina
Makerere University Institute of Environment and Natural Resources

Author:  Tjørnehøj, Kirsten (Cwisno: 39266)
Technical University of Denmark
Email:

Author:  Alexandersen, Søren (Cwisno: 39440)
Technical University of Denmark
Abstract: Diagnosis and control of Foot-and-mouth disease virus (FMDV) requires rapid and sensitive diagnostic tests. Two antibody enzyme-linked immunosorbent assay (ELISA) kits, Ceditest FMDV-NS for the detection of antibodies against the nonstructural proteins of all FMDV serotypes and Ceditest FMDV type O for the detection of antibodies against serotype O, were evaluated under African endemic conditions where the presence of multiple serotypes and the use of nonpurified vaccines complicate serological diagnosis. Serum samples from 218 African buffalo, 758 cattle, 304 goats, and 88 sheep were tested using both kits, and selected samples were tested not only in serotype-specific ELISAs for antibodies against primarily FMDV serotype O, but also against other serotypes. The FMDV-NS assay detected far more positive samples (93%) than the FMDV type O assay (30%) in buffalo (P <0.05), with predominant antibodies against the South African Territories (SAT) serotypes, while the seroprevalence was generally comparable in cattle with antibodies against serotype O elicited by infection and/or vaccination. However, some districts had higher seroprevalence using the FMDV type O assay indicating vaccination without infection, while 1 cattle herd with antibodies against the SAT serotypes had far more positive samples (85%) using the FMDV-NS versus the FMDV type O (10%), consistent with the latter test’s lower sensitivity for antibodies against SAT serotypes. Based on the current investigation, the FMDV type O ELISA may be limited by the presence of SAT serotypes. The FMD NS assay worked well as a screening test for antibodies against all FMDV serotypes present in Uganda; however, as long as nonpurified vaccines are applied in the region, this test cannot be used to differentiate between vaccinated and infected animals.
Published: in journal: Journal of Veterinary Diagnostic Investigation (ISSN: 1040-6387) (DOI: http://dx.doi.org/10.1177/1040638711435807), vol: 24, issue: 2, pages: 270-276, 2012
DOI:
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