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Title: Measurement of bacterial gene expression in vivo by laser capture microdissection and quantitative real-time RT-PCR
Type: Journal articleJournal article
Participant(s):
Author:  Schou, Kirstine Klitgaard (Cwisno: 44255)
Technical University of Denmark
Email:

Author:  Jensen, Tim Kåre (Cwisno: 39468)
Technical University of Denmark
Email:

Author:  Angen, Øystein (Cwisno: 39395)
Technical University of Denmark

Author:  Boye, Mette (Cwisno: 7574)
Technical University of Denmark
Email:

Abstract: Due to the relative small number of bacterial pathogens present in an infected host, exploration of pathogen gene expression in vivo is challenging. This study reports the development of a protocol for quantifying bacterial gene expression in vivo in Actinobacillus pleuropneumoniae using laser capture microdissection and real-time quantitative RT-PCR.
Published: in journal: Journal of Microbiological Methods (ISSN: 0167-7012) (DOI: http://dx.doi.org/10.1016/j.mimet.2006.12.003), vol: 69, issue: 2, pages: 414-416, 2007
DOI:
See the publication in DTU Orbit See the publication in DTU Orbit

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