Title: Diversity and zoonotic potential of rotaviruses in swine and cattle across Europe.
Type: Journal articleJournal article
Participant(s):
Forfatter:  Midgley, Sofie E.
Statens Serum Institut, Department of Virology

Forfatter:  Bányai, Krisztián
Hungarian Academy of Sciences, Veterinary Medical Research Institute

Forfatter:  Buesa, Javier
University of Valencia

Forfatter:  Halaihel, Nabil
University of Zaragoza, Faculty of Veterinary Medicine

Author:  Hjulsager, Charlotte Kristiane (Cwisno: 39095)
Technical University of Denmark
Email:

Forfatter:  Jakab, Ferenc
University of Pécs, Institute of Biology

Forfatter:  Kaplon, Jeérôme
Centre National de Référence des Virus Entériques, France

Author:  Larsen, Lars Erik (Cwisno: 39284)
Technical University of Denmark
Email:

Forfatter:  Monini, Marina
Centre National de Référence des Virus Entériques, France

Forfatter:  Poljšak-Prijatelj, Mateja
Istituto Superiore di Sanità, Department of Veterinary Public Health and Food Safety

Forfatter:  Pothier, Pierre
University of Pécs, Institute of Biology

Forfatter:  Ruggeri, Franco M.
Centre National de Référence des Virus Entériques, France

Forfatter:  Steyer, Andrej
University of Ljubljana, Faculty of Medicine

Forfatter:  Koopmans, Marion
National Institute for Public Health and Environment, The Netherlands

Forfatter:  Böttiger, Blenda
Statens Serum Institut, Department of Virology
Abstract: Group A rotaviruses can infect both humans and animals. Individual rotavirus strains can occasionally cross species barriers and might hereby contribute to the emergence of new genotypes in heterologous hosts. The incidence and impact of zoonotic rotavirus are not well defined, and one reason for this is a lack of data about strains circulating in suspected reservoir animal hosts. In this study we report the incidence, genetic diversity, and molecular epidemiology of rotaviruses detected in domestic cattle and swine in 6 European countries. From 2003 to 2007, 1101 and more than 2000 faecal specimens were collected from swine and cattle, both healthy and diarrhoeic, and tested for rotaviruses. Viruses from positive stools were genotyped and a subset of strains was characterized by nucleotide sequencing and phylogenetic analysis of the VP7 (G) and VP4 (P) genes. Rotaviruses were detected in 43% of bovine samples and in 14% of porcine samples. In cattle, 10 different combinations of G and P types were identified and the most common strains were G6P[11] and G6P[5]. In swine, the number of identified G–P combinations was higher (n = 21), however, no single combination was predominant across Europe. Newly described genotype specificities, P[27] and P[32], were identified in swine. When compared at the nucleotide sequence level, the identified porcine rotavirus strains and contemporary human strains grouped together phylogenetically, whereas bovine rotavirus strains formed separate clades. These data demonstrate large genetic diversity of porcine and bovine rotavirus strains across Europe, and suggest that livestock herds may serve as potential reservoirs for human infections.
Published: in journal: Veterinary Microbiology (ISSN: 0378-1135) (DOI: http://dx.doi.org/10.1016/j.vetmic.2011.10.027), vol: 156, issue: 3-4, pages: 238-245, 2012
DOI:
See the publication in DTU Orbit See the publication in DTU Orbit